ssr_primer
Primer Details for TRINITY_DN11749_c3_g1_i1 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGACATGATGATGGCGCTA | AGTGAAAACTCAAGTGCAAAGGT | Start: 20 |
| Tm | 59.965 | 59.236 | End: 224 |
| Size | 20 | 23 | 205 |
| Primer 2 details |
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| Sequence | GGGACATGATGATGGCGCTA | GTGAAAACTCAAGTGCAAAGGT | Start: 20 |
| Tm | 59.965 | 57.89 | End: 223 |
| Size | 20 | 22 | 204 |
| Primer 3 details |
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| Sequence | TCAAGTGCGCCTCTTGATCC | AGTGAAAACTCAAGTGCAAAGGT | Start: 66 |
| Tm | 60.392 | 59.236 | End: 224 |
| Size | 20 | 23 | 159 |
| Primer 4 details |
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| Sequence | CAAGTGCGCCTCTTGATCCT | AGTGAAAACTCAAGTGCAAAGGT | Start: 67 |
| Tm | 60.392 | 59.236 | End: 224 |
| Size | 20 | 23 | 158 |
| Primer 5 details |
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| Sequence | TGGCGCTATAGGGGATGTCA | AGTGAAAACTCAAGTGCAAAGGT | Start: 32 |
| Tm | 60.471 | 59.236 | End: 224 |
| Size | 20 | 23 | 193 |
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