ssr_primer
Primer Details for TRINITY_DN11749_c0_g8_i1 |
|---|
| (A)32: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCGTTTCGAATCTCGCC | CGACGGATGCGATCACAAAC | Start: 450 |
| Tm | 60.179 | 59.975 | End: 721 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | CCGGTGCTCCTCTTTCAACT | GGCGAGATTCGAAACGGAGA | Start: 255 |
| Tm | 59.965 | 60.179 | End: 469 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | GTTTGTGATCGCATCCGTCG | CGCCGTCGTTGTTTGAATCA | Start: 702 |
| Tm | 59.975 | 59.766 | End: 911 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | TCTCCGTTTCGAATCTCGCC | CCTCCTCGTTCTGTAGTCGC | Start: 450 |
| Tm | 60.179 | 59.901 | End: 693 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | CCTTCGGTTCCCCTTCCATC | GGCGAGATTCGAAACGGAGA | Start: 303 |
| Tm | 60.107 | 60.179 | End: 469 |
| Size | 20 | 20 | 167 |
(CTT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCGTTTCGAATCTCGCC | CGACGGATGCGATCACAAAC | Start: 450 |
| Tm | 60.179 | 59.975 | End: 721 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | CCGGTGCTCCTCTTTCAACT | GGCGAGATTCGAAACGGAGA | Start: 255 |
| Tm | 59.965 | 60.179 | End: 469 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | GTTTGTGATCGCATCCGTCG | CGCCGTCGTTGTTTGAATCA | Start: 702 |
| Tm | 59.975 | 59.766 | End: 911 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | TCTCCGTTTCGAATCTCGCC | CCTCCTCGTTCTGTAGTCGC | Start: 450 |
| Tm | 60.179 | 59.901 | End: 693 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | CCTTCGGTTCCCCTTCCATC | GGCGAGATTCGAAACGGAGA | Start: 303 |
| Tm | 60.107 | 60.179 | End: 469 |
| Size | 20 | 20 | 167 |
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