ssr_primer
Primer Details for TRINITY_DN11749_c0_g1_i2 |
|---|
| (GCC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGTTTGTTGCACCGATT | GCGGTTATCGAAACACGTCG | Start: 322 |
| Tm | 59.97 | 59.977 | End: 570 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | ACCGATTAGCTTTGACGCCA | GCGGTTATCGAAACACGTCG | Start: 335 |
| Tm | 60.037 | 59.977 | End: 570 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | CCATTAAGGGTGGGGCCTTT | GCGGTTATCGAAACACGTCG | Start: 352 |
| Tm | 59.957 | 59.977 | End: 570 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | AAAGCAAGGGATTCCGTCGT | CTTGGTGGTGCAACAATGGG | Start: 1709 |
| Tm | 59.964 | 59.966 | End: 1939 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | TGAGTGGCGGAGTAGTGAGA | CTTGGTGGTGCAACAATGGG | Start: 1671 |
| Tm | 59.962 | 59.966 | End: 1939 |
| Size | 20 | 20 | 269 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGTTTGTTGCACCGATT | GCGGTTATCGAAACACGTCG | Start: 322 |
| Tm | 59.97 | 59.977 | End: 570 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | ACCGATTAGCTTTGACGCCA | GCGGTTATCGAAACACGTCG | Start: 335 |
| Tm | 60.037 | 59.977 | End: 570 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | CCATTAAGGGTGGGGCCTTT | GCGGTTATCGAAACACGTCG | Start: 352 |
| Tm | 59.957 | 59.977 | End: 570 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | AAAGCAAGGGATTCCGTCGT | CTTGGTGGTGCAACAATGGG | Start: 1709 |
| Tm | 59.964 | 59.966 | End: 1939 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | TGAGTGGCGGAGTAGTGAGA | CTTGGTGGTGCAACAATGGG | Start: 1671 |
| Tm | 59.962 | 59.966 | End: 1939 |
| Size | 20 | 20 | 269 |
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