ssr_primer
Primer Details for TRINITY_DN11736_c1_g2_i18 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGTTGGGAGTAAGGCCC | ATTGTCTCTTGGCTCGGTGG | Start: 115 |
| Tm | 60.034 | 60.036 | End: 366 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | GGGAAGTTGGGAGTAAGGCC | ATTGTCTCTTGGCTCGGTGG | Start: 114 |
| Tm | 60.034 | 60.036 | End: 366 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | GGCTCTTTCTGGCCATGACT | GACATGTGGGCTTGCCTTTG | Start: 866 |
| Tm | 60.035 | 60.038 | End: 1131 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | CAAAGGCAAGCCCACATGTC | CAGCAGGGAGAGGGAAGTTG | Start: 1112 |
| Tm | 60.038 | 60.036 | End: 1325 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TCCATCGTTTTGCGCCAAAG | CAGCAGGGAGAGGGAAGTTG | Start: 1097 |
| Tm | 60.04 | 60.036 | End: 1325 |
| Size | 20 | 20 | 229 |
(A)13gagaaaag(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGTTGGGAGTAAGGCCC | ATTGTCTCTTGGCTCGGTGG | Start: 115 |
| Tm | 60.034 | 60.036 | End: 366 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | GGGAAGTTGGGAGTAAGGCC | ATTGTCTCTTGGCTCGGTGG | Start: 114 |
| Tm | 60.034 | 60.036 | End: 366 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | GGCTCTTTCTGGCCATGACT | GACATGTGGGCTTGCCTTTG | Start: 866 |
| Tm | 60.035 | 60.038 | End: 1131 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | CAAAGGCAAGCCCACATGTC | CAGCAGGGAGAGGGAAGTTG | Start: 1112 |
| Tm | 60.038 | 60.036 | End: 1325 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TCCATCGTTTTGCGCCAAAG | CAGCAGGGAGAGGGAAGTTG | Start: 1097 |
| Tm | 60.04 | 60.036 | End: 1325 |
| Size | 20 | 20 | 229 |
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