Primer Details for TRINITY_DN11723_c7_g1_i1 | |||
|---|---|---|---|
| (A)20: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAATTCTTTTGAAATCAGGACA | TCCTTTTCCTAGCCACAAGTACC | Start: 11 |
| Tm | 57.025 | 59.991 | End: 133 |
| Size | 24 | 23 | 123 |
| Primer 2 details | |||
| Sequence | TGGAATTCTTTTGAAATCAGGACA | TGTTCCTTTTCCTAGCCACAAGT | Start: 11 |
| Tm | 57.025 | 60.118 | End: 136 |
| Size | 24 | 23 | 126 |
| Primer 3 details | |||
| Sequence | TGGAATTCTTTTGAAATCAGGACA | CCTTTTCCTAGCCACAAGTACCA | Start: 11 |
| Tm | 57.025 | 60.245 | End: 132 |
| Size | 24 | 23 | 122 |
| Primer 4 details | |||
| Sequence | TGGAATTCTTTTGAAATCAGGACA | CCTTTTCCTAGCCACAAGTACC | Start: 11 |
| Tm | 57.025 | 58.655 | End: 132 |
| Size | 24 | 22 | 122 |
| Primer 5 details | |||
| Sequence | TGGAATTCTTTTGAAATCAGGACAA | TCCTTTTCCTAGCCACAAGTACC | Start: 11 |
| Tm | 57.641 | 59.991 | End: 133 |
| Size | 25 | 23 | 123 |
