ssr_primer
Primer Details for TRINITY_DN11714_c0_g2_i4 |
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| (AAG)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCGAGCAAGACGAATCTT | ACAGCGGTGTTGACGGTATT | Start: 3233 |
| Tm | 60.037 | 59.966 | End: 3374 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
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| Sequence | TTGGCGAGCAAGACGAATCT | ACAGCGGTGTTGACGGTATT | Start: 3232 |
| Tm | 60.037 | 59.966 | End: 3374 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
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| Sequence | GCCTTACACTGTTGCCTCCT | CACTGGAAGGGCTTCTGGAG | Start: 717 |
| Tm | 59.963 | 60.036 | End: 874 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
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| Sequence | AGCCTTACACTGTTGCCTCC | CACTGGAAGGGCTTCTGGAG | Start: 716 |
| Tm | 59.963 | 60.036 | End: 874 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
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| Sequence | GGAAAACGGGCCCTTCCTTA | AAAGGAGGGGAGAAATGGCG | Start: 220 |
| Tm | 59.961 | 60.034 | End: 447 |
| Size | 20 | 20 | 228 |
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