ssr_primer
Primer Details for TRINITY_DN11695_c0_g1_i2 |
|---|
| (TA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGAGCATGATAGACCG | GGGGAGCATTGGTCGAATCA | Start: 894 |
| Tm | 59.966 | 60.107 | End: 1172 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | TCTCTTGCCACGAGAGAGGA | TGTTGGCAAGCCCTTAACCA | Start: 98 |
| Tm | 59.962 | 60.106 | End: 353 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | ACTACTTCCATGGCGGCAAA | GAACCGCACACCCAAACAAA | Start: 1554 |
| Tm | 59.962 | 59.826 | End: 1695 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | ACTACTTCCATGGCGGCAAA | TTTGAACCGCACACCCAAAC | Start: 1554 |
| Tm | 59.962 | 59.826 | End: 1698 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | AACTACTTCCATGGCGGCAA | GAACCGCACACCCAAACAAA | Start: 1553 |
| Tm | 59.962 | 59.826 | End: 1695 |
| Size | 20 | 20 | 143 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGAGCATGATAGACCG | GGGGAGCATTGGTCGAATCA | Start: 894 |
| Tm | 59.966 | 60.107 | End: 1172 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | TCTCTTGCCACGAGAGAGGA | TGTTGGCAAGCCCTTAACCA | Start: 98 |
| Tm | 59.962 | 60.106 | End: 353 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | ACTACTTCCATGGCGGCAAA | GAACCGCACACCCAAACAAA | Start: 1554 |
| Tm | 59.962 | 59.826 | End: 1695 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | ACTACTTCCATGGCGGCAAA | TTTGAACCGCACACCCAAAC | Start: 1554 |
| Tm | 59.962 | 59.826 | End: 1698 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | AACTACTTCCATGGCGGCAA | GAACCGCACACCCAAACAAA | Start: 1553 |
| Tm | 59.962 | 59.826 | End: 1695 |
| Size | 20 | 20 | 143 |
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