Primer Details for TRINITY_DN11673_c0_g1_i1 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCTCACAAATTCAAACTTCACA | AAGGAAGGGTAGATACAAAAGAGT | Start: 92 |
| Tm | 59.815 | 57.046 | End: 208 |
| Size | 25 | 24 | 117 |
| Primer 2 details |
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| Sequence | ACACCTCACAAATTCAAACTTCACA | AAAGGAAGGGTAGATACAAAAGAGT | Start: 92 |
| Tm | 59.815 | 57.669 | End: 209 |
| Size | 25 | 25 | 118 |
| Primer 3 details |
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| Sequence | CACCTCACAAATTCAAACTTCACA | AAGGAAGGGTAGATACAAAAGAGT | Start: 93 |
| Tm | 58.35 | 57.046 | End: 208 |
| Size | 24 | 24 | 116 |
| Primer 4 details |
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| Sequence | ACACCTCACAAATTCAAACTTCAC | AAGGAAGGGTAGATACAAAAGAGT | Start: 92 |
| Tm | 58.349 | 57.046 | End: 208 |
| Size | 24 | 24 | 117 |
| Primer 5 details |
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| Sequence | TCCAAACATACACCTCACAAATTCA | AAGGAAGGGTAGATACAAAAGAGT | Start: 83 |
| Tm | 59.17 | 57.046 | End: 208 |
| Size | 25 | 24 | 126 |
