ssr_primer
Primer Details for TRINITY_DN11656_c1_g4_i5 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCAGTTGGACAGGAAGCG | CTCTCACTCCCCTCGACTGA | Start: 1594 |
| Tm | 59.966 | 60.035 | End: 1821 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | ACTCAGTTGGACAGGAAGCG | TCTCTCACTCCCCTCGACTG | Start: 1594 |
| Tm | 59.966 | 60.035 | End: 1822 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | TTCTACAACCAGACCACGGC | TTCCGAACACCGTCGTAGTG | Start: 1042 |
| Tm | 59.966 | 60.04 | End: 1163 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | CGGCCAATTTTGAAGGGTGG | TTCCGAACACCGTCGTAGTG | Start: 997 |
| Tm | 60.037 | 60.04 | End: 1163 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACTGCTCGGCAATGTCTTCA | CGACAACTGCACAAGCCAAA | Start: 2140 |
| Tm | 59.965 | 59.9 | End: 2409 |
| Size | 20 | 20 | 270 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCAGTTGGACAGGAAGCG | CTCTCACTCCCCTCGACTGA | Start: 1594 |
| Tm | 59.966 | 60.035 | End: 1821 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | ACTCAGTTGGACAGGAAGCG | TCTCTCACTCCCCTCGACTG | Start: 1594 |
| Tm | 59.966 | 60.035 | End: 1822 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | TTCTACAACCAGACCACGGC | TTCCGAACACCGTCGTAGTG | Start: 1042 |
| Tm | 59.966 | 60.04 | End: 1163 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | CGGCCAATTTTGAAGGGTGG | TTCCGAACACCGTCGTAGTG | Start: 997 |
| Tm | 60.037 | 60.04 | End: 1163 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACTGCTCGGCAATGTCTTCA | CGACAACTGCACAAGCCAAA | Start: 2140 |
| Tm | 59.965 | 59.9 | End: 2409 |
| Size | 20 | 20 | 270 |
(T)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCAGTTGGACAGGAAGCG | CTCTCACTCCCCTCGACTGA | Start: 1594 |
| Tm | 59.966 | 60.035 | End: 1821 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | ACTCAGTTGGACAGGAAGCG | TCTCTCACTCCCCTCGACTG | Start: 1594 |
| Tm | 59.966 | 60.035 | End: 1822 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | TTCTACAACCAGACCACGGC | TTCCGAACACCGTCGTAGTG | Start: 1042 |
| Tm | 59.966 | 60.04 | End: 1163 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | CGGCCAATTTTGAAGGGTGG | TTCCGAACACCGTCGTAGTG | Start: 997 |
| Tm | 60.037 | 60.04 | End: 1163 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACTGCTCGGCAATGTCTTCA | CGACAACTGCACAAGCCAAA | Start: 2140 |
| Tm | 59.965 | 59.9 | End: 2409 |
| Size | 20 | 20 | 270 |
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