ssr_primer
Primer Details for TRINITY_DN11638_c1_g1_i1 |
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| (AT)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTGCACTTTACAATCATGGT | AGCCAAGGAAAGGGTTATGTGT | Start: 13 |
| Tm | 58.908 | 59.89 | End: 281 |
| Size | 22 | 22 | 269 |
| Primer 2 details |
|---|
| Sequence | CCCTGCACTTTACAATCATGGTG | AGCCAAGGAAAGGGTTATGTGT | Start: 13 |
| Tm | 60.119 | 59.89 | End: 281 |
| Size | 23 | 22 | 269 |
| Primer 3 details |
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| Sequence | TGGGGTAGACCTCCACAAAA | GCTCTTCAACTTATGGCCTTTGT | Start: 292 |
| Tm | 58.178 | 59.492 | End: 417 |
| Size | 20 | 23 | 126 |
| Primer 4 details |
|---|
| Sequence | TGGGGTAGACCTCCACAAAA | ACAAAGCTCTTCAACTTATGGCC | Start: 292 |
| Tm | 58.178 | 59.492 | End: 422 |
| Size | 20 | 23 | 131 |
| Primer 5 details |
|---|
| Sequence | TCCCTGCACTTTACAATCATGGT | AGCCAAGGAAAGGGTTATGTGT | Start: 12 |
| Tm | 60.245 | 59.89 | End: 281 |
| Size | 23 | 22 | 270 |
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