ssr_primer
Primer Details for TRINITY_DN11622_c1_g2_i1 |
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| (T)10cttttccttttttttaggaaaccagcctcattaataaaacgaat(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGAAAGCACTGCCACTC | AGAGGCAGCCAATTCCTCAC | Start: 473 |
| Tm | 60.109 | 60.035 | End: 741 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
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| Sequence | GCATGAAAGCACTGCCACTC | ACAGAGGCAGCCAATTCCTC | Start: 473 |
| Tm | 60.109 | 60.035 | End: 743 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
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| Sequence | GCATGAAAGCACTGCCACTC | CAGAGGCAGCCAATTCCTCA | Start: 473 |
| Tm | 60.109 | 60.035 | End: 742 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
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| Sequence | CAGGAGAAAGTGCGCAGAGA | GAGTGGCAGTGCTTTCATGC | Start: 354 |
| Tm | 60.038 | 60.109 | End: 492 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
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| Sequence | TGAAGCAGGCCATGAACCTT | AGAGGCAGCCAATTCCTCAC | Start: 598 |
| Tm | 59.887 | 60.035 | End: 741 |
| Size | 20 | 20 | 144 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGAAAGCACTGCCACTC | AGAGGCAGCCAATTCCTCAC | Start: 473 |
| Tm | 60.109 | 60.035 | End: 741 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
|---|
| Sequence | GCATGAAAGCACTGCCACTC | ACAGAGGCAGCCAATTCCTC | Start: 473 |
| Tm | 60.109 | 60.035 | End: 743 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GCATGAAAGCACTGCCACTC | CAGAGGCAGCCAATTCCTCA | Start: 473 |
| Tm | 60.109 | 60.035 | End: 742 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CAGGAGAAAGTGCGCAGAGA | GAGTGGCAGTGCTTTCATGC | Start: 354 |
| Tm | 60.038 | 60.109 | End: 492 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TGAAGCAGGCCATGAACCTT | AGAGGCAGCCAATTCCTCAC | Start: 598 |
| Tm | 59.887 | 60.035 | End: 741 |
| Size | 20 | 20 | 144 |
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