Primer Details for TRINITY_DN11622_c10_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGCTGAGTGAATAGAGACC | TCCTAGTGTTAAAGGAAATTAGGGT | Start: 36 |
| Tm | 59.933 | 57.422 | End: 135 |
| Size | 23 | 25 | 100 |
| Primer 2 details |
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| Sequence | CATCGCTGAGTGAATAGAGACCA | TTCCTAGTGTTAAAGGAAATTAGGGT | Start: 37 |
| Tm | 59.933 | 58.008 | End: 136 |
| Size | 23 | 26 | 100 |
| Primer 3 details |
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| Sequence | ACATCGCTGAGTGAATAGAGACC | TCCTAGTGTTAAAGGAAATTAGGGTT | Start: 36 |
| Tm | 59.933 | 58.008 | End: 135 |
| Size | 23 | 26 | 100 |
| Primer 4 details |
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| Sequence | ACATCGCTGAGTGAATAGAGACC | TTCCTAGTGTTAAAGGAAATTAGGGT | Start: 36 |
| Tm | 59.933 | 58.008 | End: 136 |
| Size | 23 | 26 | 101 |
| Primer 5 details |
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| Sequence | CATCGCTGAGTGAATAGAGACCA | TTCCTAGTGTTAAAGGAAATTAGGGTT | Start: 37 |
| Tm | 59.933 | 58.55 | End: 136 |
| Size | 23 | 27 | 100 |
