ssr_primer
Primer Details for TRINITY_DN11598_c4_g1_i1 |
|---|
| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATAGCAACCATCCCCCGAG | TGGGCCAAATAATCGCCACT | Start: 987 |
| Tm | 59.965 | 60.034 | End: 1240 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | GAGATAGCAACCATCCCCCG | TGGGCCAAATAATCGCCACT | Start: 985 |
| Tm | 59.965 | 60.034 | End: 1240 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | TTGCGAGGGTCCTAGGATGA | CCAGGCCACCCACTGTAATT | Start: 1965 |
| Tm | 60.033 | 59.96 | End: 2094 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | TGAGAAACCTTGCGAGGGTC | CCAGGCCACCCACTGTAATT | Start: 1956 |
| Tm | 59.965 | 59.96 | End: 2094 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | AGGCCTACTTCCACCAGGAT | CCAGGCCACCCACTGTAATT | Start: 1896 |
| Tm | 59.955 | 59.96 | End: 2094 |
| Size | 20 | 20 | 199 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATAGCAACCATCCCCCGAG | TGGGCCAAATAATCGCCACT | Start: 987 |
| Tm | 59.965 | 60.034 | End: 1240 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | GAGATAGCAACCATCCCCCG | TGGGCCAAATAATCGCCACT | Start: 985 |
| Tm | 59.965 | 60.034 | End: 1240 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | TTGCGAGGGTCCTAGGATGA | CCAGGCCACCCACTGTAATT | Start: 1965 |
| Tm | 60.033 | 59.96 | End: 2094 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | TGAGAAACCTTGCGAGGGTC | CCAGGCCACCCACTGTAATT | Start: 1956 |
| Tm | 59.965 | 59.96 | End: 2094 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | AGGCCTACTTCCACCAGGAT | CCAGGCCACCCACTGTAATT | Start: 1896 |
| Tm | 59.955 | 59.96 | End: 2094 |
| Size | 20 | 20 | 199 |
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