ssr_primer
Primer Details for TRINITY_DN11585_c6_g2_i5 |
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| (T)12cggcatggtaggtaagc(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCTGTCGAAGTGAACCAG | TGGGATATGTGGAGGAGCCA | Start: 3539 |
| Tm | 59.966 | 60.03 | End: 3754 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
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| Sequence | GTAATGGAGGGGACGGCAAA | CTGGTTCACTTCGACAGCCT | Start: 3391 |
| Tm | 60.035 | 59.966 | End: 3558 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
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| Sequence | TCCCTGATTTTGCTGGACCC | TTTGCCGTCCCCTCCATTAC | Start: 3220 |
| Tm | 59.96 | 60.035 | End: 3410 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
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| Sequence | CCCTGACCAATGAGGCTTGT | AGGGGAGAAAGGGCTGGTAT | Start: 2092 |
| Tm | 59.961 | 59.954 | End: 2250 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | TGAACCAGGTTTCCGCAAGT | TGGGATATGTGGAGGAGCCA | Start: 3551 |
| Tm | 60.107 | 60.03 | End: 3754 |
| Size | 20 | 20 | 204 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCTGTCGAAGTGAACCAG | TGGGATATGTGGAGGAGCCA | Start: 3539 |
| Tm | 59.966 | 60.03 | End: 3754 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GTAATGGAGGGGACGGCAAA | CTGGTTCACTTCGACAGCCT | Start: 3391 |
| Tm | 60.035 | 59.966 | End: 3558 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | TCCCTGATTTTGCTGGACCC | TTTGCCGTCCCCTCCATTAC | Start: 3220 |
| Tm | 59.96 | 60.035 | End: 3410 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | CCCTGACCAATGAGGCTTGT | AGGGGAGAAAGGGCTGGTAT | Start: 2092 |
| Tm | 59.961 | 59.954 | End: 2250 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | TGAACCAGGTTTCCGCAAGT | TGGGATATGTGGAGGAGCCA | Start: 3551 |
| Tm | 60.107 | 60.03 | End: 3754 |
| Size | 20 | 20 | 204 |
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