ssr_primer
Primer Details for TRINITY_DN11576_c3_g1_i2 |
|---|
| (GCC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCCCTTGTGGAACTCCA | ATTGGGCTACCGGTGGAAAG | Start: 560 |
| Tm | 60.105 | 60.034 | End: 806 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | AAGGAATTGGGCTACCGGTG | Start: 560 |
| Tm | 60.105 | 60.034 | End: 811 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | AATGCGTCGGGGAAGGAATT | Start: 560 |
| Tm | 60.105 | 60.034 | End: 823 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | GAATTGGGCTACCGGTGGAA | Start: 560 |
| Tm | 60.105 | 60.035 | End: 808 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | GGGGAAGGAATTGGGCTACC | Start: 560 |
| Tm | 60.105 | 60.106 | End: 815 |
| Size | 20 | 20 | 256 |
(AAAC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCCCTTGTGGAACTCCA | ATTGGGCTACCGGTGGAAAG | Start: 560 |
| Tm | 60.105 | 60.034 | End: 806 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | AAGGAATTGGGCTACCGGTG | Start: 560 |
| Tm | 60.105 | 60.034 | End: 811 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | AATGCGTCGGGGAAGGAATT | Start: 560 |
| Tm | 60.105 | 60.034 | End: 823 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | GAATTGGGCTACCGGTGGAA | Start: 560 |
| Tm | 60.105 | 60.035 | End: 808 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | GGGGAAGGAATTGGGCTACC | Start: 560 |
| Tm | 60.105 | 60.106 | End: 815 |
| Size | 20 | 20 | 256 |
(A)16gcaaatgggggtgttttaacaatcatgtttggtcaattccaggaaggaaataaatatt(A)10gaaaag(A)10gtgatttaaccaaaacagagaagtactatatg(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCCCTTGTGGAACTCCA | ATTGGGCTACCGGTGGAAAG | Start: 560 |
| Tm | 60.105 | 60.034 | End: 806 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | AAGGAATTGGGCTACCGGTG | Start: 560 |
| Tm | 60.105 | 60.034 | End: 811 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | AATGCGTCGGGGAAGGAATT | Start: 560 |
| Tm | 60.105 | 60.034 | End: 823 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | GAATTGGGCTACCGGTGGAA | Start: 560 |
| Tm | 60.105 | 60.035 | End: 808 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TCTCCCCTTGTGGAACTCCA | GGGGAAGGAATTGGGCTACC | Start: 560 |
| Tm | 60.105 | 60.106 | End: 815 |
| Size | 20 | 20 | 256 |
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