ssr_primer
Primer Details for TRINITY_DN11575_c2_g2_i3 |
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| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGGTTGACTGCCACACAA | TTCTGGGCCAAATTTGCAGC | Start: 2532 |
| Tm | 60.035 | 59.965 | End: 2798 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
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| Sequence | AATCTTGCAGACGTGGGGAG | AAAGTTGCCGAAATTGCCCC | Start: 661 |
| Tm | 60.036 | 59.965 | End: 887 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
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| Sequence | CCCAGTGTCCATTCTGGCTT | TTGTGTGGCAGTCAACCAGT | Start: 2388 |
| Tm | 59.961 | 60.035 | End: 2551 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
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| Sequence | AATCTTGCAGACGTGGGGAG | TGTTGAATGTGCCCGACAGA | Start: 661 |
| Tm | 60.036 | 59.892 | End: 938 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
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| Sequence | GCCAGTTGCTTTGCACGTAA | AGCTTTGCTTTGGTTTGGCC | Start: 3586 |
| Tm | 59.97 | 60.179 | End: 3689 |
| Size | 20 | 20 | 104 |
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