Primer Details for TRINITY_DN11575_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGGAAATTTGGCAGAGCA | GGAAATTTCCTAATTCCTCCCCT | Start: 164 |
| Tm | 58 | 57.856 | End: 276 |
| Size | 20 | 23 | 113 |
| Primer 2 details | |||
| Sequence | AGTGGAAATTTGGCAGAGCA | TCCTAATTCCTCCCCTAAATTCCA | Start: 164 |
| Tm | 58 | 58.698 | End: 269 |
| Size | 20 | 24 | 106 |
| Primer 3 details | |||
| Sequence | TGAGTCCATGACTTTTTAGTGGT | TGCTCTGCCAAATTTCCACT | Start: 35 |
| Tm | 57.695 | 58 | End: 183 |
| Size | 23 | 20 | 149 |
| Primer 4 details | |||
| Sequence | AGTGGAAATTTGGCAGAGCAA | GGAAATTTCCTAATTCCTCCCCT | Start: 164 |
| Tm | 58.681 | 57.856 | End: 276 |
| Size | 21 | 23 | 113 |
| Primer 5 details | |||
| Sequence | CGTAGTCTACATTTATAGGGGGAGG | ACCGTTATGCAAGCAACATGA | Start: 465 |
| Tm | 59.582 | 58.841 | End: 589 |
| Size | 25 | 21 | 125 |
