ssr_primer
Primer Details for TRINITY_DN11569_c1_g1_i14 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGCCTCATGGACTCTGAT | ATGGCAAGCGACTTGAGACA | Start: 2302 |
| Tm | 60.03 | 59.965 | End: 2524 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
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| Sequence | CCTTCGTGCAGTTGGAAAGC | GCTTCTTCAGAGCAGGCTCA | Start: 385 |
| Tm | 60.04 | 60.037 | End: 659 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
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| Sequence | TCAGCCCTAGCACTCACTCT | GAAGGTCGAGGAGGTGTTGG | Start: 159 |
| Tm | 59.96 | 60.037 | End: 389 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
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| Sequence | TTAGCGGTCGTCTTGGTCAC | AGAGTGAGTGCTAGGGCTGA | Start: 38 |
| Tm | 60.039 | 59.96 | End: 178 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
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| Sequence | TCAGCCCTAGCACTCACTCT | GCTTTCCAACTGCACGAAGG | Start: 159 |
| Tm | 59.96 | 60.04 | End: 404 |
| Size | 20 | 20 | 246 |
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