ssr_primer
Primer Details for TRINITY_DN11557_c0_g2_i4 |
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| (T)29gatagaatcaacaacttttattaagaacaaaaaggaaagaatacaaaagcatacaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTCAGCTCAAACGGTGG | ACCAGCATAAAGAGCCCACC | Start: 1386 |
| Tm | 59.966 | 60.034 | End: 1615 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | ACTCTCAGCTCAAACGGTGG | CCAGCATAAAGAGCCCACCA | Start: 1386 |
| Tm | 59.966 | 60.034 | End: 1614 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GGGTGTCCTTGAAGAGCCTC | CCACCGTTTGAGCTGAGAGT | Start: 1172 |
| Tm | 60.036 | 59.966 | End: 1405 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | ATACACCCCACGAAACCACC | GAGGCTCTTCAAGGACACCC | Start: 956 |
| Tm | 59.963 | 60.036 | End: 1191 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | GCTACACGAAGAGGGCCAAT | ACCAGCATAAAGAGCCCACC | Start: 1354 |
| Tm | 60.108 | 60.034 | End: 1615 |
| Size | 20 | 20 | 262 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTCAGCTCAAACGGTGG | ACCAGCATAAAGAGCCCACC | Start: 1386 |
| Tm | 59.966 | 60.034 | End: 1615 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | ACTCTCAGCTCAAACGGTGG | CCAGCATAAAGAGCCCACCA | Start: 1386 |
| Tm | 59.966 | 60.034 | End: 1614 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GGGTGTCCTTGAAGAGCCTC | CCACCGTTTGAGCTGAGAGT | Start: 1172 |
| Tm | 60.036 | 59.966 | End: 1405 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | ATACACCCCACGAAACCACC | GAGGCTCTTCAAGGACACCC | Start: 956 |
| Tm | 59.963 | 60.036 | End: 1191 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | GCTACACGAAGAGGGCCAAT | ACCAGCATAAAGAGCCCACC | Start: 1354 |
| Tm | 60.108 | 60.034 | End: 1615 |
| Size | 20 | 20 | 262 |
(TTAT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTCAGCTCAAACGGTGG | ACCAGCATAAAGAGCCCACC | Start: 1386 |
| Tm | 59.966 | 60.034 | End: 1615 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | ACTCTCAGCTCAAACGGTGG | CCAGCATAAAGAGCCCACCA | Start: 1386 |
| Tm | 59.966 | 60.034 | End: 1614 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GGGTGTCCTTGAAGAGCCTC | CCACCGTTTGAGCTGAGAGT | Start: 1172 |
| Tm | 60.036 | 59.966 | End: 1405 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | ATACACCCCACGAAACCACC | GAGGCTCTTCAAGGACACCC | Start: 956 |
| Tm | 59.963 | 60.036 | End: 1191 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | GCTACACGAAGAGGGCCAAT | ACCAGCATAAAGAGCCCACC | Start: 1354 |
| Tm | 60.108 | 60.034 | End: 1615 |
| Size | 20 | 20 | 262 |
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