ssr_primer
Primer Details for TRINITY_DN11555_c3_g1_i4 |
|---|
| (AC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTAGGCGCTTAACCGTGGT | TCAAGCCGACGAGACGAAAA | Start: 1346 |
| Tm | 59.965 | 59.969 | End: 1524 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | TTTTCGTCTCGTCGGCTTGA | GTTGGATCTTTCTCGCCCCA | Start: 1505 |
| Tm | 59.969 | 60.035 | End: 1770 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | TGGGGCGAGAAAGATCCAAC | TCTCTGTATCCCCACCCTGG | Start: 1751 |
| Tm | 60.035 | 60.031 | End: 1921 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GCTACTCCGGAGCTTTGGTT | TCAAGCCGACGAGACGAAAA | Start: 1319 |
| Tm | 60.036 | 59.969 | End: 1524 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
|---|
| Sequence | ACACTGCGGCATGTACTTGA | TCGGGTGAGGAGTGCAGATA | Start: 531 |
| Tm | 59.965 | 60.034 | End: 672 |
| Size | 20 | 20 | 142 |
(A)25: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTAGGCGCTTAACCGTGGT | TCAAGCCGACGAGACGAAAA | Start: 1346 |
| Tm | 59.965 | 59.969 | End: 1524 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | TTTTCGTCTCGTCGGCTTGA | GTTGGATCTTTCTCGCCCCA | Start: 1505 |
| Tm | 59.969 | 60.035 | End: 1770 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | TGGGGCGAGAAAGATCCAAC | TCTCTGTATCCCCACCCTGG | Start: 1751 |
| Tm | 60.035 | 60.031 | End: 1921 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GCTACTCCGGAGCTTTGGTT | TCAAGCCGACGAGACGAAAA | Start: 1319 |
| Tm | 60.036 | 59.969 | End: 1524 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
|---|
| Sequence | ACACTGCGGCATGTACTTGA | TCGGGTGAGGAGTGCAGATA | Start: 531 |
| Tm | 59.965 | 60.034 | End: 672 |
| Size | 20 | 20 | 142 |
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