ssr_primer
Primer Details for TRINITY_DN11533_c0_g1_i5 |
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| (A)32ctcttccaaacatagctttaaatgcgagttggttgatctc(CTT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCGGTCCGGCCATATTGA | CCCAGGCAATCCTTTTGCAC | Start: 929 |
| Tm | 59.965 | 60.037 | End: 1144 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
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| Sequence | TTGGGGAATTGGGTTTGCCT | CAAGCAAAGTCCCAACTGCC | Start: 435 |
| Tm | 60.105 | 59.967 | End: 562 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | TTGGGGAATTGGGTTTGCCT | CCAAGCAAAGTCCCAACTGC | Start: 435 |
| Tm | 60.105 | 59.967 | End: 563 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
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| Sequence | CATCGGTCCGGCCATATTGA | CGACTGAATCCCCAGGCAAT | Start: 929 |
| Tm | 59.965 | 60.107 | End: 1154 |
| Size | 20 | 20 | 226 |
| Primer 5 details |
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| Sequence | TTGGGGAATTGGGTTTGCCT | CTTGGCCAATCAATGCTCCG | Start: 435 |
| Tm | 60.105 | 59.898 | End: 592 |
| Size | 20 | 20 | 158 |
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