Primer Details for TRINITY_DN11517_c3_g1_i1 | |||
|---|---|---|---|
| (A)19: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGGGTGCTTACTGCTTGGT | AGAGACTCTTTTTCCACTTTCGA | Start: 155 |
| Tm | 59.521 | 57.722 | End: 254 |
| Size | 20 | 23 | 100 |
| Primer 2 details | |||
| Sequence | AGGGTGCTTACTGCTTGGTT | AAGAGACTCTTTTTCCACTTTCGA | Start: 156 |
| Tm | 59.521 | 58.329 | End: 255 |
| Size | 20 | 24 | 100 |
| Primer 3 details | |||
| Sequence | AAGGGTGCTTACTGCTTGGT | AAGAGACTCTTTTTCCACTTTCGA | Start: 155 |
| Tm | 59.521 | 58.329 | End: 255 |
| Size | 20 | 24 | 101 |
| Primer 4 details | |||
| Sequence | AAAGGGTGCTTACTGCTTGGT | GAGACTCTTTTTCCACTTTCGATCG | Start: 154 |
| Tm | 60.133 | 60.164 | End: 253 |
| Size | 21 | 25 | 100 |
| Primer 5 details | |||
| Sequence | AAGGGTGCTTACTGCTTGGT | AGAGACTCTTTTTCCACTTTCGAT | Start: 155 |
| Tm | 59.521 | 58.141 | End: 254 |
| Size | 20 | 24 | 100 |
