Primer Details for TRINITY_DN11496_c5_g1_i1 | |||
|---|---|---|---|
| (TTA)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACTTTGATGTTCCATTGTGGA | CGAATGATCGTGATGGAATACCG | Start: 159 |
| Tm | 59.108 | 59.635 | End: 310 |
| Size | 24 | 23 | 152 |
| Primer 2 details | |||
| Sequence | CAACTTTGATGTTCCATTGTGGA | CGAATGATCGTGATGGAATACCG | Start: 160 |
| Tm | 57.801 | 59.635 | End: 310 |
| Size | 23 | 23 | 151 |
| Primer 3 details | |||
| Sequence | TCAACTTTGATGTTCCATTGTGG | CGAATGATCGTGATGGAATACCG | Start: 159 |
| Tm | 57.801 | 59.635 | End: 310 |
| Size | 23 | 23 | 152 |
| Primer 4 details | |||
| Sequence | TCAACTTTGATGTTCCATTGTGGA | TGATCGTGATGGAATACCGTT | Start: 159 |
| Tm | 59.108 | 57.188 | End: 306 |
| Size | 24 | 21 | 148 |
| Primer 5 details | |||
| Sequence | TTCAACTTTGATGTTCCATTGTGGA | CGAATGATCGTGATGGAATACCG | Start: 158 |
| Tm | 59.638 | 59.635 | End: 310 |
| Size | 25 | 23 | 153 |
