ssr_primer
Primer Details for TRINITY_DN11450_c3_g7_i1 |
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| (TCT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGTTGCTGTGACATTGGC | TCCACTTACCCATCCCCTCT | Start: 804 |
| Tm | 60.319 | 59.277 | End: 1041 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
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| Sequence | AGCAATCTCGGGACAAAGCA | AGAGAGATGGAGGGGTTGTCA | Start: 45 |
| Tm | 59.963 | 59.921 | End: 209 |
| Size | 20 | 21 | 165 |
| Primer 3 details |
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| Sequence | AGTCTAGCAAACGTCGGTCG | AGAGAGATGGAGGGGTTGTCA | Start: 12 |
| Tm | 60.11 | 59.921 | End: 209 |
| Size | 20 | 21 | 198 |
| Primer 4 details |
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| Sequence | TCTAGCAAACGTCGGTCGAC | AGAGAGATGGAGGGGTTGTCA | Start: 14 |
| Tm | 60.11 | 59.921 | End: 209 |
| Size | 20 | 21 | 196 |
| Primer 5 details |
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| Sequence | GTCTAGCAAACGTCGGTCGA | AGAGAGATGGAGGGGTTGTCA | Start: 13 |
| Tm | 60.11 | 59.921 | End: 209 |
| Size | 20 | 21 | 197 |
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