ssr_primer
Primer Details for TRINITY_DN11445_c1_g2_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCCGCCAACAAGCAAATC | GGGCTTGGGCAGTAGTTCTT | Start: 1066 |
| Tm | 59.967 | 59.962 | End: 1259 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | GAACCCGCCAACAAGCAAAT | GGGCTTGGGCAGTAGTTCTT | Start: 1065 |
| Tm | 59.967 | 59.962 | End: 1259 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | TCATGCCAATGCCTGTGTCT | ATTTGCTTGTTGGCGGGTTC | Start: 862 |
| Tm | 59.961 | 59.967 | End: 1084 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | TCATGCCAATGCCTGTGTCT | GATTTGCTTGTTGGCGGGTT | Start: 862 |
| Tm | 59.961 | 59.967 | End: 1085 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AAGAACTACTGCCCAAGCCC | AGACCCAGGTGCGAAAACAA | Start: 1240 |
| Tm | 59.962 | 60.107 | End: 1417 |
| Size | 20 | 20 | 178 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCCGCCAACAAGCAAATC | GGGCTTGGGCAGTAGTTCTT | Start: 1066 |
| Tm | 59.967 | 59.962 | End: 1259 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | GAACCCGCCAACAAGCAAAT | GGGCTTGGGCAGTAGTTCTT | Start: 1065 |
| Tm | 59.967 | 59.962 | End: 1259 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | TCATGCCAATGCCTGTGTCT | ATTTGCTTGTTGGCGGGTTC | Start: 862 |
| Tm | 59.961 | 59.967 | End: 1084 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | TCATGCCAATGCCTGTGTCT | GATTTGCTTGTTGGCGGGTT | Start: 862 |
| Tm | 59.961 | 59.967 | End: 1085 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AAGAACTACTGCCCAAGCCC | AGACCCAGGTGCGAAAACAA | Start: 1240 |
| Tm | 59.962 | 60.107 | End: 1417 |
| Size | 20 | 20 | 178 |
(T)11cttagtttctactctgctttctctttcacagcaaaggaataagattggtataattataacagatatgctgtgttcaaaattcgaagggaaac(A)26: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCCGCCAACAAGCAAATC | GGGCTTGGGCAGTAGTTCTT | Start: 1066 |
| Tm | 59.967 | 59.962 | End: 1259 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | GAACCCGCCAACAAGCAAAT | GGGCTTGGGCAGTAGTTCTT | Start: 1065 |
| Tm | 59.967 | 59.962 | End: 1259 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | TCATGCCAATGCCTGTGTCT | ATTTGCTTGTTGGCGGGTTC | Start: 862 |
| Tm | 59.961 | 59.967 | End: 1084 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | TCATGCCAATGCCTGTGTCT | GATTTGCTTGTTGGCGGGTT | Start: 862 |
| Tm | 59.961 | 59.967 | End: 1085 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AAGAACTACTGCCCAAGCCC | AGACCCAGGTGCGAAAACAA | Start: 1240 |
| Tm | 59.962 | 60.107 | End: 1417 |
| Size | 20 | 20 | 178 |
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