ssr_primer
Primer Details for TRINITY_DN11414_c0_g3_i4 |
|---|
| (A)10tcagttttgtaactcgtaggtaataaccatcattaaatttggacctgattcaaacctaatttaaaaattctcactataatcatcaaatgttgggt(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGCTGCATGGTGCTTTTG | TGGGTTTGGCCAAGAGTGTT | Start: 3128 |
| Tm | 59.967 | 60.033 | End: 3256 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | AGTGCTGCATGGTGCTTTTG | GATGAGGATGCGGGTGAGAG | Start: 3128 |
| Tm | 59.967 | 59.967 | End: 3285 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | GGTGTGCTTGTTTGCAGAGG | CCTTGCTTCTGGGTTCCGAT | Start: 4161 |
| Tm | 59.969 | 60.035 | End: 4280 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | GGGTGTGCTTGTTTGCAGAG | CCTTGCTTCTGGGTTCCGAT | Start: 4160 |
| Tm | 59.969 | 60.035 | End: 4280 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TTTTGCCATTCAGGTGCAGC | TGGGTTTGGCCAAGAGTGTT | Start: 3143 |
| Tm | 59.966 | 60.033 | End: 3256 |
| Size | 20 | 20 | 114 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGCTGCATGGTGCTTTTG | TGGGTTTGGCCAAGAGTGTT | Start: 3128 |
| Tm | 59.967 | 60.033 | End: 3256 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | AGTGCTGCATGGTGCTTTTG | GATGAGGATGCGGGTGAGAG | Start: 3128 |
| Tm | 59.967 | 59.967 | End: 3285 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | GGTGTGCTTGTTTGCAGAGG | CCTTGCTTCTGGGTTCCGAT | Start: 4161 |
| Tm | 59.969 | 60.035 | End: 4280 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | GGGTGTGCTTGTTTGCAGAG | CCTTGCTTCTGGGTTCCGAT | Start: 4160 |
| Tm | 59.969 | 60.035 | End: 4280 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TTTTGCCATTCAGGTGCAGC | TGGGTTTGGCCAAGAGTGTT | Start: 3143 |
| Tm | 59.966 | 60.033 | End: 3256 |
| Size | 20 | 20 | 114 |
(GCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGCTGCATGGTGCTTTTG | TGGGTTTGGCCAAGAGTGTT | Start: 3128 |
| Tm | 59.967 | 60.033 | End: 3256 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | AGTGCTGCATGGTGCTTTTG | GATGAGGATGCGGGTGAGAG | Start: 3128 |
| Tm | 59.967 | 59.967 | End: 3285 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | GGTGTGCTTGTTTGCAGAGG | CCTTGCTTCTGGGTTCCGAT | Start: 4161 |
| Tm | 59.969 | 60.035 | End: 4280 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | GGGTGTGCTTGTTTGCAGAG | CCTTGCTTCTGGGTTCCGAT | Start: 4160 |
| Tm | 59.969 | 60.035 | End: 4280 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TTTTGCCATTCAGGTGCAGC | TGGGTTTGGCCAAGAGTGTT | Start: 3143 |
| Tm | 59.966 | 60.033 | End: 3256 |
| Size | 20 | 20 | 114 |
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