Primer Details for TRINITY_DN11399_c4_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGTGCAATTGATGTTGAGATGA | ACGTGTGTCTCACCTTCATCC | Start: 56 |
| Tm | 58.749 | 59.999 | End: 161 |
| Size | 24 | 21 | 106 |
| Primer 2 details | |||
| Sequence | AGTGCAATTGATGTTGAGATGATGT | ACGTGTGTCTCACCTTCATCC | Start: 58 |
| Tm | 59.582 | 59.999 | End: 161 |
| Size | 25 | 21 | 104 |
| Primer 3 details | |||
| Sequence | GAGTGCAATTGATGTTGAGATGA | ACGTGTGTCTCACCTTCATCC | Start: 57 |
| Tm | 57.444 | 59.999 | End: 161 |
| Size | 23 | 21 | 105 |
| Primer 4 details | |||
| Sequence | AGAGTGCAATTGATGTTGAGATG | ACGTGTGTCTCACCTTCATCC | Start: 56 |
| Tm | 57.437 | 59.999 | End: 161 |
| Size | 23 | 21 | 106 |
| Primer 5 details | |||
| Sequence | AGAGTGCAATTGATGTTGAGATGA | GTGTGTCTCACCTTCATCCCA | Start: 56 |
| Tm | 58.749 | 59.652 | End: 159 |
| Size | 24 | 21 | 104 |
