ssr_primer
Primer Details for TRINITY_DN11361_c1_g5_i3 |
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| (T)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGGGTGAGGAAGAGACGA | GAGACTGAGAGCGAATCCCG | Start: 3435 |
| Tm | 60.034 | 59.97 | End: 3562 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | AAACTGGCACAACGGAATGC | TATTGGAGGCAGAGTCGGGA | Start: 950 |
| Tm | 59.968 | 60.033 | End: 1175 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
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| Sequence | ATCGGGTGAGGAAGAGACGA | GGCGGTGGATTGAATGATGC | Start: 3435 |
| Tm | 60.034 | 59.969 | End: 3583 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
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| Sequence | GGAAGAGACGAGGGTTGTGG | GAGACTGAGAGCGAATCCCG | Start: 3444 |
| Tm | 60.037 | 59.97 | End: 3562 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
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| Sequence | AGAACGGCGACTTTGAGAGG | GAGACTGAGAGCGAATCCCG | Start: 3311 |
| Tm | 60.038 | 59.97 | End: 3562 |
| Size | 20 | 20 | 252 |
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