ssr_primer
Primer Details for TRINITY_DN11359_c4_g5_i4 |
|---|
| (AAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGATTGACTCACCACAGC | GCAATCGTTCGATTCCGTGG | Start: 672 |
| Tm | 60.037 | 59.974 | End: 782 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | CCACGGAATCGAACGATTGC | TCCTCGGATCAACCTCTGGT | Start: 763 |
| Tm | 59.974 | 59.959 | End: 881 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CCACGGAATCGAACGATTGC | CTTGCCGTTCACCGTTGAAG | Start: 763 |
| Tm | 59.974 | 60.041 | End: 1034 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | TTCAACCCGACGCCATTACA | TCCTCGGATCAACCTCTGGT | Start: 726 |
| Tm | 59.965 | 59.959 | End: 881 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | TCCGATTGACTCACCACAGC | TCCTCGGATCAACCTCTGGT | Start: 672 |
| Tm | 60.037 | 59.959 | End: 881 |
| Size | 20 | 20 | 210 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGATTGACTCACCACAGC | GCAATCGTTCGATTCCGTGG | Start: 672 |
| Tm | 60.037 | 59.974 | End: 782 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | CCACGGAATCGAACGATTGC | TCCTCGGATCAACCTCTGGT | Start: 763 |
| Tm | 59.974 | 59.959 | End: 881 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CCACGGAATCGAACGATTGC | CTTGCCGTTCACCGTTGAAG | Start: 763 |
| Tm | 59.974 | 60.041 | End: 1034 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | TTCAACCCGACGCCATTACA | TCCTCGGATCAACCTCTGGT | Start: 726 |
| Tm | 59.965 | 59.959 | End: 881 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | TCCGATTGACTCACCACAGC | TCCTCGGATCAACCTCTGGT | Start: 672 |
| Tm | 60.037 | 59.959 | End: 881 |
| Size | 20 | 20 | 210 |
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