ssr_primer
Primer Details for TRINITY_DN11321_c6_g1_i3 |
|---|
| (TTA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGCCCTCTATCCTTATT | CCACGACCTCCCTGACTCTA | Start: 403 |
| Tm | 60.037 | 60.034 | End: 581 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | ATGTGGAGAAACTGCCGGAG | AATAAGGATAGAGGGCGCGC | Start: 290 |
| Tm | 60.036 | 60.037 | End: 422 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAGTCTCGGCCATTGAAGCA | AATAAGGATAGAGGGCGCGC | Start: 215 |
| Tm | 59.963 | 60.037 | End: 422 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | CTCCTTCCTGGTTGAGGCTG | TAGTGGTCTTCGGCGTGTTC | Start: 1966 |
| Tm | 60.036 | 60.039 | End: 2218 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCGCGCCCTCTATCCTTATT | TACCTGAGACGAGCCTTGGA | Start: 403 |
| Tm | 60.037 | 59.961 | End: 639 |
| Size | 20 | 20 | 237 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGCCCTCTATCCTTATT | CCACGACCTCCCTGACTCTA | Start: 403 |
| Tm | 60.037 | 60.034 | End: 581 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | ATGTGGAGAAACTGCCGGAG | AATAAGGATAGAGGGCGCGC | Start: 290 |
| Tm | 60.036 | 60.037 | End: 422 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAGTCTCGGCCATTGAAGCA | AATAAGGATAGAGGGCGCGC | Start: 215 |
| Tm | 59.963 | 60.037 | End: 422 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | CTCCTTCCTGGTTGAGGCTG | TAGTGGTCTTCGGCGTGTTC | Start: 1966 |
| Tm | 60.036 | 60.039 | End: 2218 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCGCGCCCTCTATCCTTATT | TACCTGAGACGAGCCTTGGA | Start: 403 |
| Tm | 60.037 | 59.961 | End: 639 |
| Size | 20 | 20 | 237 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGCCCTCTATCCTTATT | CCACGACCTCCCTGACTCTA | Start: 403 |
| Tm | 60.037 | 60.034 | End: 581 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | ATGTGGAGAAACTGCCGGAG | AATAAGGATAGAGGGCGCGC | Start: 290 |
| Tm | 60.036 | 60.037 | End: 422 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAGTCTCGGCCATTGAAGCA | AATAAGGATAGAGGGCGCGC | Start: 215 |
| Tm | 59.963 | 60.037 | End: 422 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | CTCCTTCCTGGTTGAGGCTG | TAGTGGTCTTCGGCGTGTTC | Start: 1966 |
| Tm | 60.036 | 60.039 | End: 2218 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCGCGCCCTCTATCCTTATT | TACCTGAGACGAGCCTTGGA | Start: 403 |
| Tm | 60.037 | 59.961 | End: 639 |
| Size | 20 | 20 | 237 |
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