ssr_primers

ssr_primer

Primer Details for TRINITY_DN11299_c1_g1_i7
(A)10: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(TA)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(TA)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(CTG)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(AT)11: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(TTC)8tttctcttcaccatctctttg(A)10: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(CAG)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(ACA)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182
(TTTC)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCCCCTACCCGCAAATCAAC	ACAGTGGCTCAGTGCTTCTC	Start: 4949
Tm	60.035	59.965	End: 5123
Size	20	20	175
Primer 2 details
Sequence	TCCCCTACCCGCAAATCAAC	GACAGTGGCTCAGTGCTTCT	Start: 4949
Tm	60.035	59.965	End: 5124
Size	20	20	176
Primer 3 details
Sequence	TCCAACCACGCTTTCTCCTC	GGGTTAGGTGAGCTTGCTGT	Start: 3305
Tm	59.965	59.963	End: 3441
Size	20	20	137
Primer 4 details
Sequence	GTCTTTTCCCCTACCCGCAA	ACAGTGGCTCAGTGCTTCTC	Start: 4943
Tm	59.963	59.965	End: 5123
Size	20	20	181
Primer 5 details
Sequence	GTCTTTTCCCCTACCCGCAA	GACAGTGGCTCAGTGCTTCT	Start: 4943
Tm	59.963	59.965	End: 5124
Size	20	20	182

CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

Primer Details for TRINITY_DN11299_c1_g1_i7

ICAR Data Use Licence