ssr_primer
Primer Details for TRINITY_DN11293_c1_g7_i2 |
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| (A)10cagcaaaagtgaagagaaaaaccaa(AACG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTGGTGGATGTGAGCTC | CTCGTACAAGAAACCCCGCT | Start: 1918 |
| Tm | 60.036 | 60.038 | End: 2181 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
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| Sequence | AAGCTGGTGGATGTGAGCTC | TCTCGTACAAGAAACCCCGC | Start: 1918 |
| Tm | 60.036 | 60.038 | End: 2182 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
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| Sequence | ATCTGGTTCCGGGTCTCTGA | GAGCTCACATCCACCAGCTT | Start: 1796 |
| Tm | 59.959 | 60.036 | End: 1937 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | ACTGGGGATGGCGTGAATTT | TCAGAGACCCGGAACCAGAT | Start: 1645 |
| Tm | 59.96 | 59.959 | End: 1815 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
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| Sequence | TTCCGGGTTTCTGGTTCAGG | GAGCTCACATCCACCAGCTT | Start: 1694 |
| Tm | 59.89 | 60.036 | End: 1937 |
| Size | 20 | 20 | 244 |
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