ssr_primer
Primer Details for TRINITY_DN11272_c7_g5_i1 |
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| (T)10cgggagggtggaggggagatcttgaaagatt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACCAGCCAACCAAGGTTT | AAGATCTCCCCTCCACCCTC | Start: 620 |
| Tm | 60.033 | 60.031 | End: 869 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
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| Sequence | GCAACCAGGCTAATCACCCT | AAACCTTGGTTGGCTGGTGA | Start: 472 |
| Tm | 60.034 | 60.033 | End: 639 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
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| Sequence | GGTAGGCCAGCCTTAGCAAA | ACACCCTGAGGACTGATCGA | Start: 1008 |
| Tm | 60.034 | 59.96 | End: 1280 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
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| Sequence | TTTGGTAGGCCAGCCTTAGC | ACACCCTGAGGACTGATCGA | Start: 1005 |
| Tm | 60.034 | 59.96 | End: 1280 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | TCCCTTGTTTCTCACCAGCC | AAGATCTCCCCTCCACCCTC | Start: 609 |
| Tm | 59.89 | 60.031 | End: 869 |
| Size | 20 | 20 | 261 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACCAGCCAACCAAGGTTT | AAGATCTCCCCTCCACCCTC | Start: 620 |
| Tm | 60.033 | 60.031 | End: 869 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | GCAACCAGGCTAATCACCCT | AAACCTTGGTTGGCTGGTGA | Start: 472 |
| Tm | 60.034 | 60.033 | End: 639 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | GGTAGGCCAGCCTTAGCAAA | ACACCCTGAGGACTGATCGA | Start: 1008 |
| Tm | 60.034 | 59.96 | End: 1280 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TTTGGTAGGCCAGCCTTAGC | ACACCCTGAGGACTGATCGA | Start: 1005 |
| Tm | 60.034 | 59.96 | End: 1280 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
|---|
| Sequence | TCCCTTGTTTCTCACCAGCC | AAGATCTCCCCTCCACCCTC | Start: 609 |
| Tm | 59.89 | 60.031 | End: 869 |
| Size | 20 | 20 | 261 |
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