ssr_primer
Primer Details for TRINITY_DN11261_c0_g3_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCTCCGCCAATTCCTCC | GACGGTCTCGGATCGAACTC | Start: 605 |
| Tm | 60.034 | 59.972 | End: 832 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | TTTCCTCCGCCAATTCCTCC | GACATGGACGGTCTCGGATC | Start: 605 |
| Tm | 60.034 | 59.969 | End: 838 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CCAACCTCACCCTCACTGTC | GGAGGAATTGGCGGAGGAAA | Start: 511 |
| Tm | 59.965 | 60.034 | End: 624 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | TTTCCTCCGCCAATTCCTCC | ATCGAACTCCCCGACTAGCT | Start: 605 |
| Tm | 60.034 | 60.107 | End: 821 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | TTTCCTCCGCCAATTCCTCC | TCCTCCTCCTCGTCGTACTC | Start: 605 |
| Tm | 60.034 | 59.822 | End: 778 |
| Size | 20 | 20 | 174 |
(CCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCTCCGCCAATTCCTCC | GACGGTCTCGGATCGAACTC | Start: 605 |
| Tm | 60.034 | 59.972 | End: 832 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | TTTCCTCCGCCAATTCCTCC | GACATGGACGGTCTCGGATC | Start: 605 |
| Tm | 60.034 | 59.969 | End: 838 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CCAACCTCACCCTCACTGTC | GGAGGAATTGGCGGAGGAAA | Start: 511 |
| Tm | 59.965 | 60.034 | End: 624 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | TTTCCTCCGCCAATTCCTCC | ATCGAACTCCCCGACTAGCT | Start: 605 |
| Tm | 60.034 | 60.107 | End: 821 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | TTTCCTCCGCCAATTCCTCC | TCCTCCTCCTCGTCGTACTC | Start: 605 |
| Tm | 60.034 | 59.822 | End: 778 |
| Size | 20 | 20 | 174 |
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