Primer Details for TRINITY_DN11258_c2_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAAAGATAGAGCAAAGAAATGGG | TGGTAGTGAGCTTTGCTTCTT | Start: 102 |
| Tm | 59.932 | 57.508 | End: 245 |
| Size | 25 | 21 | 144 |
| Primer 2 details |
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| Sequence | GGGAAAGATAGAGCAAAGAAATGGG | ATGGTAGTGAGCTTTGCTTCT | Start: 102 |
| Tm | 59.932 | 57.29 | End: 246 |
| Size | 25 | 21 | 145 |
| Primer 3 details |
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| Sequence | GGGAAAGATAGAGCAAAGAAATGGG | TGGTAGTGAGCTTTGCTTCTTTC | Start: 102 |
| Tm | 59.932 | 59.185 | End: 245 |
| Size | 25 | 23 | 144 |
| Primer 4 details |
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| Sequence | GGGAAAGATAGAGCAAAGAAATGGG | TGGTAGTGAGCTTTGCTTCTTT | Start: 102 |
| Tm | 59.932 | 58.179 | End: 245 |
| Size | 25 | 22 | 144 |
| Primer 5 details |
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| Sequence | GGGAAAGATAGAGCAAAGAAATGGG | ATGGTAGTGAGCTTTGCTTCTT | Start: 102 |
| Tm | 59.932 | 57.974 | End: 246 |
| Size | 25 | 22 | 145 |
