ssr_primer
Primer Details for TRINITY_DN11254_c0_g1_i1 |
|---|
| (GAGAAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGTCTGTGTCGTCTTCA | GAGGAAACTGACGGAGCCAA | Start: 425 |
| Tm | 59.97 | 59.965 | End: 663 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TTACGTCGCAAGGAACCGAA | ACCCACCAATTAGCGACGTT | Start: 2562 |
| Tm | 59.968 | 59.964 | End: 2720 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TTACGTCGCAAGGAACCGAA | GCAGTTTCTAGCGGGGAGTT | Start: 2562 |
| Tm | 59.968 | 60.036 | End: 2824 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | TTCCTACTGGGCTGCACTTG | TCAACAAGGGGAGTCGAAGC | Start: 1613 |
| Tm | 59.963 | 59.965 | End: 1826 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TTCCTACTGGGCTGCACTTG | CGAAACCCCATGCTTCCTCT | Start: 1613 |
| Tm | 59.963 | 60.035 | End: 1859 |
| Size | 20 | 20 | 247 |
(TGT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGTCTGTGTCGTCTTCA | GAGGAAACTGACGGAGCCAA | Start: 425 |
| Tm | 59.97 | 59.965 | End: 663 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TTACGTCGCAAGGAACCGAA | ACCCACCAATTAGCGACGTT | Start: 2562 |
| Tm | 59.968 | 59.964 | End: 2720 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TTACGTCGCAAGGAACCGAA | GCAGTTTCTAGCGGGGAGTT | Start: 2562 |
| Tm | 59.968 | 60.036 | End: 2824 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | TTCCTACTGGGCTGCACTTG | TCAACAAGGGGAGTCGAAGC | Start: 1613 |
| Tm | 59.963 | 59.965 | End: 1826 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TTCCTACTGGGCTGCACTTG | CGAAACCCCATGCTTCCTCT | Start: 1613 |
| Tm | 59.963 | 60.035 | End: 1859 |
| Size | 20 | 20 | 247 |
(TAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGTCTGTGTCGTCTTCA | GAGGAAACTGACGGAGCCAA | Start: 425 |
| Tm | 59.97 | 59.965 | End: 663 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TTACGTCGCAAGGAACCGAA | ACCCACCAATTAGCGACGTT | Start: 2562 |
| Tm | 59.968 | 59.964 | End: 2720 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TTACGTCGCAAGGAACCGAA | GCAGTTTCTAGCGGGGAGTT | Start: 2562 |
| Tm | 59.968 | 60.036 | End: 2824 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | TTCCTACTGGGCTGCACTTG | TCAACAAGGGGAGTCGAAGC | Start: 1613 |
| Tm | 59.963 | 59.965 | End: 1826 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TTCCTACTGGGCTGCACTTG | CGAAACCCCATGCTTCCTCT | Start: 1613 |
| Tm | 59.963 | 60.035 | End: 1859 |
| Size | 20 | 20 | 247 |
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