ssr_primer
Primer Details for TRINITY_DN11250_c5_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGGCTTGGGGGAATTTCCA | GACGTCCAAAGTGATCCCCA | Start: 48 |
| Tm | 60.252 | 59.676 | End: 208 |
| Size | 20 | 20 | 161 |
| Primer 2 details |
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| Sequence | AGGCTTGGGGGAATTTCCAC | GACGTCCAAAGTGATCCCCA | Start: 49 |
| Tm | 60.252 | 59.676 | End: 208 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
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| Sequence | ACAGGCTTGGGGGAATTTCC | GACGTCCAAAGTGATCCCCA | Start: 47 |
| Tm | 60.252 | 59.676 | End: 208 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
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| Sequence | CAGGCTTGGGGGAATTTCCA | CTCTTGTCCCTCGCCCTTAG | Start: 48 |
| Tm | 60.252 | 59.538 | End: 296 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
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| Sequence | AGGCTTGGGGGAATTTCCAC | CTCTTGTCCCTCGCCCTTAG | Start: 49 |
| Tm | 60.252 | 59.538 | End: 296 |
| Size | 20 | 20 | 248 |
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