ssr_primer
Primer Details for TRINITY_DN11232_c1_g2_i4 |
|---|
| (AG)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGCGCCAATCCGAATTC | CTTACCGCCGGTAGTAGCAG | Start: 84 |
| Tm | 59.972 | 59.97 | End: 359 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CAGCTGTGGTAGTTGTCGGT | Start: 84 |
| Tm | 59.972 | 59.966 | End: 342 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CTGCTACTACCGGCGGTAAG | GCAGGGAGTGGGAAGTTACC | Start: 340 |
| Tm | 59.97 | 60.035 | End: 478 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CTGTGGTAGTTGTCGGTCCC | Start: 84 |
| Tm | 59.972 | 60.038 | End: 339 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGTGGGGTATCGTTGAAGC | TTCCCTATCTCGAGCACCCA | Start: 1539 |
| Tm | 60.036 | 60.033 | End: 1723 |
| Size | 20 | 20 | 185 |
(TA)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGCGCCAATCCGAATTC | CTTACCGCCGGTAGTAGCAG | Start: 84 |
| Tm | 59.972 | 59.97 | End: 359 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CAGCTGTGGTAGTTGTCGGT | Start: 84 |
| Tm | 59.972 | 59.966 | End: 342 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CTGCTACTACCGGCGGTAAG | GCAGGGAGTGGGAAGTTACC | Start: 340 |
| Tm | 59.97 | 60.035 | End: 478 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CTGTGGTAGTTGTCGGTCCC | Start: 84 |
| Tm | 59.972 | 60.038 | End: 339 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGTGGGGTATCGTTGAAGC | TTCCCTATCTCGAGCACCCA | Start: 1539 |
| Tm | 60.036 | 60.033 | End: 1723 |
| Size | 20 | 20 | 185 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGCGCCAATCCGAATTC | CTTACCGCCGGTAGTAGCAG | Start: 84 |
| Tm | 59.972 | 59.97 | End: 359 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CAGCTGTGGTAGTTGTCGGT | Start: 84 |
| Tm | 59.972 | 59.966 | End: 342 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CTGCTACTACCGGCGGTAAG | GCAGGGAGTGGGAAGTTACC | Start: 340 |
| Tm | 59.97 | 60.035 | End: 478 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CTGTGGTAGTTGTCGGTCCC | Start: 84 |
| Tm | 59.972 | 60.038 | End: 339 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGTGGGGTATCGTTGAAGC | TTCCCTATCTCGAGCACCCA | Start: 1539 |
| Tm | 60.036 | 60.033 | End: 1723 |
| Size | 20 | 20 | 185 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGCGCCAATCCGAATTC | CTTACCGCCGGTAGTAGCAG | Start: 84 |
| Tm | 59.972 | 59.97 | End: 359 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CAGCTGTGGTAGTTGTCGGT | Start: 84 |
| Tm | 59.972 | 59.966 | End: 342 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CTGCTACTACCGGCGGTAAG | GCAGGGAGTGGGAAGTTACC | Start: 340 |
| Tm | 59.97 | 60.035 | End: 478 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CTGTGGTAGTTGTCGGTCCC | Start: 84 |
| Tm | 59.972 | 60.038 | End: 339 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGTGGGGTATCGTTGAAGC | TTCCCTATCTCGAGCACCCA | Start: 1539 |
| Tm | 60.036 | 60.033 | End: 1723 |
| Size | 20 | 20 | 185 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGCGCCAATCCGAATTC | CTTACCGCCGGTAGTAGCAG | Start: 84 |
| Tm | 59.972 | 59.97 | End: 359 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CAGCTGTGGTAGTTGTCGGT | Start: 84 |
| Tm | 59.972 | 59.966 | End: 342 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CTGCTACTACCGGCGGTAAG | GCAGGGAGTGGGAAGTTACC | Start: 340 |
| Tm | 59.97 | 60.035 | End: 478 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CTGTGGTAGTTGTCGGTCCC | Start: 84 |
| Tm | 59.972 | 60.038 | End: 339 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGTGGGGTATCGTTGAAGC | TTCCCTATCTCGAGCACCCA | Start: 1539 |
| Tm | 60.036 | 60.033 | End: 1723 |
| Size | 20 | 20 | 185 |
(TA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGCGCCAATCCGAATTC | CTTACCGCCGGTAGTAGCAG | Start: 84 |
| Tm | 59.972 | 59.97 | End: 359 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CAGCTGTGGTAGTTGTCGGT | Start: 84 |
| Tm | 59.972 | 59.966 | End: 342 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CTGCTACTACCGGCGGTAAG | GCAGGGAGTGGGAAGTTACC | Start: 340 |
| Tm | 59.97 | 60.035 | End: 478 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GACTGCGCCAATCCGAATTC | CTGTGGTAGTTGTCGGTCCC | Start: 84 |
| Tm | 59.972 | 60.038 | End: 339 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGTGGGGTATCGTTGAAGC | TTCCCTATCTCGAGCACCCA | Start: 1539 |
| Tm | 60.036 | 60.033 | End: 1723 |
| Size | 20 | 20 | 185 |
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