ssr_primer
Primer Details for TRINITY_DN11224_c1_g8_i1 |
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| (A)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTCTCTGGAACGAGGCTA | ACACGAACAGCAAAGGACCA | Start: 830 |
| Tm | 60.034 | 60.107 | End: 977 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
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| Sequence | ACGGAACCCAATTTCGGACA | AGTTTTGGGCCTGCACGATA | Start: 1660 |
| Tm | 59.891 | 59.962 | End: 1776 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
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| Sequence | CTTGGGAGCTCTCTGGAACG | ACACGAACAGCAAAGGACCA | Start: 824 |
| Tm | 60.108 | 60.107 | End: 977 |
| Size | 20 | 20 | 154 |
| Primer 4 details |
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| Sequence | AGGAAAGGGCAAGTCCAACC | AGTTTTGGGCCTGCACGATA | Start: 1589 |
| Tm | 60.179 | 59.962 | End: 1776 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
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| Sequence | GGAACGAGGCTAAGCGAGAA | ACACGAACAGCAAAGGACCA | Start: 838 |
| Tm | 59.827 | 60.107 | End: 977 |
| Size | 20 | 20 | 140 |
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