Primer Details for TRINITY_DN11224_c1_g6_i1 | |||
|---|---|---|---|
| (A)22: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCAGATTACGGCACACA | TGTATGAAGGCCTTAGAAGTTTCA | Start: 105 |
| Tm | 59.751 | 57.878 | End: 339 |
| Size | 20 | 24 | 235 |
| Primer 2 details | |||
| Sequence | ACCTCCAGATTACGGCACAC | TGTATGAAGGCCTTAGAAGTTTCA | Start: 104 |
| Tm | 59.751 | 57.878 | End: 339 |
| Size | 20 | 24 | 236 |
| Primer 3 details | |||
| Sequence | CCTCCAGATTACGGCACACA | TGTATGAAGGCCTTAGAAGTTTCAA | Start: 105 |
| Tm | 59.751 | 58.461 | End: 339 |
| Size | 20 | 25 | 235 |
| Primer 4 details | |||
| Sequence | ACCTCCAGATTACGGCACAC | TGTATGAAGGCCTTAGAAGTTTCAA | Start: 104 |
| Tm | 59.751 | 58.461 | End: 339 |
| Size | 20 | 25 | 236 |
| Primer 5 details | |||
| Sequence | TCCAGATTACGGCACACACA | TGTATGAAGGCCTTAGAAGTTTCA | Start: 107 |
| Tm | 59.318 | 57.878 | End: 339 |
| Size | 20 | 24 | 233 |
