ssr_primer
Primer Details for TRINITY_DN11224_c1_g5_i6 |
|---|
| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGAGGAGGAGGAAGAGCA | GGGCTGGAGGGTTTATTGCT | Start: 1953 |
| Tm | 60.033 | 60.033 | End: 2087 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | GCATTGACTGTTGGCTGCAA | AATCCCAGTTGGCCTCGAAG | Start: 877 |
| Tm | 59.968 | 60.035 | End: 1082 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | GGCCGATACTTCCAACCCAA | AATTGAGACACGGGAGCTGG | Start: 173 |
| Tm | 60.035 | 60.036 | End: 398 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | CCAGCTCCCGTGTCTCAATT | TTTGCAGAAGGGTCCGAGTC | Start: 379 |
| Tm | 60.036 | 59.965 | End: 529 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | AGAGGGCAAAAGAGAGCGAC | TTGGGTTGGAAGTATCGGCC | Start: 84 |
| Tm | 60.037 | 60.035 | End: 192 |
| Size | 20 | 20 | 109 |
(GGA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGAGGAGGAGGAAGAGCA | GGGCTGGAGGGTTTATTGCT | Start: 1953 |
| Tm | 60.033 | 60.033 | End: 2087 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | GCATTGACTGTTGGCTGCAA | AATCCCAGTTGGCCTCGAAG | Start: 877 |
| Tm | 59.968 | 60.035 | End: 1082 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | GGCCGATACTTCCAACCCAA | AATTGAGACACGGGAGCTGG | Start: 173 |
| Tm | 60.035 | 60.036 | End: 398 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | CCAGCTCCCGTGTCTCAATT | TTTGCAGAAGGGTCCGAGTC | Start: 379 |
| Tm | 60.036 | 59.965 | End: 529 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | AGAGGGCAAAAGAGAGCGAC | TTGGGTTGGAAGTATCGGCC | Start: 84 |
| Tm | 60.037 | 60.035 | End: 192 |
| Size | 20 | 20 | 109 |
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