ssr_primer
Primer Details for TRINITY_DN11224_c1_g2_i13 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGAAATGGGTTCAGACGG | TGGCGCGTCGATCATTATCA | Start: 488 |
| Tm | 60.037 | 59.969 | End: 722 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | CCTTGCCCTCAGGAATTGGT | GGTGAACTCGTACCCTGGTG | Start: 2360 |
| Tm | 59.96 | 60.038 | End: 2481 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | ACCTTGCCCTCAGGAATTGG | GGTGAACTCGTACCCTGGTG | Start: 2359 |
| Tm | 59.96 | 60.038 | End: 2481 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | TGATAATGATCGACGCGCCA | CTTTCCGATCAACGTCGTGC | Start: 703 |
| Tm | 59.969 | 59.907 | End: 843 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | AGTATCGGACGCAGCTTCAG | TGGCGCGTCGATCATTATCA | Start: 547 |
| Tm | 59.899 | 59.969 | End: 722 |
| Size | 20 | 20 | 176 |
(GGA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGAAATGGGTTCAGACGG | TGGCGCGTCGATCATTATCA | Start: 488 |
| Tm | 60.037 | 59.969 | End: 722 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | CCTTGCCCTCAGGAATTGGT | GGTGAACTCGTACCCTGGTG | Start: 2360 |
| Tm | 59.96 | 60.038 | End: 2481 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | ACCTTGCCCTCAGGAATTGG | GGTGAACTCGTACCCTGGTG | Start: 2359 |
| Tm | 59.96 | 60.038 | End: 2481 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | TGATAATGATCGACGCGCCA | CTTTCCGATCAACGTCGTGC | Start: 703 |
| Tm | 59.969 | 59.907 | End: 843 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | AGTATCGGACGCAGCTTCAG | TGGCGCGTCGATCATTATCA | Start: 547 |
| Tm | 59.899 | 59.969 | End: 722 |
| Size | 20 | 20 | 176 |
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