ssr_primer
Primer Details for TRINITY_DN11224_c0_g1_i2 |
|---|
| (A)13g(A)10ggaaaaaaaggaaaagaaaagaaagaaaaaacacgaaggcttggaccaaagtcctccatttcccctccttccttcttctctccgccgacgtcaccgt(CAC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTTTCTTGCTCCTGAGG | CATTTCCCCTGGGTCTGCAT | Start: 246 |
| Tm | 59.963 | 60.033 | End: 347 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | ATCCATTTGCAGGGAACGGT | TCATCTGGGTGCGGAAGAAC | Start: 1162 |
| Tm | 59.96 | 60.036 | End: 1318 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | CAGCTGTCCGTGTCACAGAT | ACCGTTCCCTGCAAATGGAT | Start: 941 |
| Tm | 60.038 | 59.96 | End: 1181 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | GACAGGGTGGTTGTTGGGAA | CATTTCCCCTGGGTCTGCAT | Start: 226 |
| Tm | 60.106 | 60.033 | End: 347 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | ACACGAAGGCTTGGACCAAA | CCTCAGGAGCAAGAAAGGCA | Start: 61 |
| Tm | 60.107 | 59.963 | End: 265 |
| Size | 20 | 20 | 205 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTTTCTTGCTCCTGAGG | CATTTCCCCTGGGTCTGCAT | Start: 246 |
| Tm | 59.963 | 60.033 | End: 347 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | ATCCATTTGCAGGGAACGGT | TCATCTGGGTGCGGAAGAAC | Start: 1162 |
| Tm | 59.96 | 60.036 | End: 1318 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | CAGCTGTCCGTGTCACAGAT | ACCGTTCCCTGCAAATGGAT | Start: 941 |
| Tm | 60.038 | 59.96 | End: 1181 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | GACAGGGTGGTTGTTGGGAA | CATTTCCCCTGGGTCTGCAT | Start: 226 |
| Tm | 60.106 | 60.033 | End: 347 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | ACACGAAGGCTTGGACCAAA | CCTCAGGAGCAAGAAAGGCA | Start: 61 |
| Tm | 60.107 | 59.963 | End: 265 |
| Size | 20 | 20 | 205 |
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