ssr_primer
Primer Details for TRINITY_DN11221_c2_g5_i1 |
|---|
| (AAT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGTGCCTTTTTCAGCG | CTCTTGGGGTGGCATCCAAT | Start: 1618 |
| Tm | 59.97 | 60.033 | End: 1822 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TTTCCGTGCCTTTTTCAGCG | TATTTCCGCTCAACCCCACC | Start: 1618 |
| Tm | 59.97 | 60.035 | End: 1786 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | ATTGGATGCCACCCCAAGAG | ATTCCACCGATGTTTCGCCT | Start: 1803 |
| Tm | 60.033 | 60.035 | End: 1945 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | AAGCGTAACTAAACCGGCGA | CGCTGAAAAAGGCACGGAAA | Start: 1429 |
| Tm | 60.038 | 59.97 | End: 1637 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATTGGATGCCACCCCAAGAG | CGACAGAAACCACCCCATGA | Start: 1803 |
| Tm | 60.033 | 59.964 | End: 1972 |
| Size | 20 | 20 | 170 |
(AG)11aaattcc(A)15gtaaaaa(AT)6tc(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGTGCCTTTTTCAGCG | CTCTTGGGGTGGCATCCAAT | Start: 1618 |
| Tm | 59.97 | 60.033 | End: 1822 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TTTCCGTGCCTTTTTCAGCG | TATTTCCGCTCAACCCCACC | Start: 1618 |
| Tm | 59.97 | 60.035 | End: 1786 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | ATTGGATGCCACCCCAAGAG | ATTCCACCGATGTTTCGCCT | Start: 1803 |
| Tm | 60.033 | 60.035 | End: 1945 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | AAGCGTAACTAAACCGGCGA | CGCTGAAAAAGGCACGGAAA | Start: 1429 |
| Tm | 60.038 | 59.97 | End: 1637 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATTGGATGCCACCCCAAGAG | CGACAGAAACCACCCCATGA | Start: 1803 |
| Tm | 60.033 | 59.964 | End: 1972 |
| Size | 20 | 20 | 170 |
(AAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGTGCCTTTTTCAGCG | CTCTTGGGGTGGCATCCAAT | Start: 1618 |
| Tm | 59.97 | 60.033 | End: 1822 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TTTCCGTGCCTTTTTCAGCG | TATTTCCGCTCAACCCCACC | Start: 1618 |
| Tm | 59.97 | 60.035 | End: 1786 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | ATTGGATGCCACCCCAAGAG | ATTCCACCGATGTTTCGCCT | Start: 1803 |
| Tm | 60.033 | 60.035 | End: 1945 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | AAGCGTAACTAAACCGGCGA | CGCTGAAAAAGGCACGGAAA | Start: 1429 |
| Tm | 60.038 | 59.97 | End: 1637 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATTGGATGCCACCCCAAGAG | CGACAGAAACCACCCCATGA | Start: 1803 |
| Tm | 60.033 | 59.964 | End: 1972 |
| Size | 20 | 20 | 170 |
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