Primer Details for TRINITY_DN11221_c1_g3_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGAAATGGGCATTTGACCC | TCCCATTTCATTTCATGAGATCTGT | Start: 48 |
| Tm | 57.585 | 58.571 | End: 148 |
| Size | 21 | 25 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTGAAATGGGCATTTGACCC | TCCCATTTCATTTCATGAGATCTGTC | Start: 48 |
| Tm | 57.585 | 59.455 | End: 148 |
| Size | 21 | 26 | 101 |
| Primer 3 details |
|---|
| Sequence | TTTGAAATGGGCATTTGACCC | CCCATTTCATTTCATGAGATCTGTCT | Start: 48 |
| Tm | 57.585 | 59.454 | End: 147 |
| Size | 21 | 26 | 100 |
| Primer 4 details |
|---|
| Sequence | TGGTTTCAAGGATATTGGACCCA | ACAAACGAGTACTCCATATTCTCT | Start: 93 |
| Tm | 59.665 | 57.223 | End: 215 |
| Size | 23 | 24 | 123 |
| Primer 5 details |
|---|
| Sequence | TTTGAAATGGGCATTTGACCC | CCCATTTCATTTCATGAGATCTGT | Start: 48 |
| Tm | 57.585 | 57.289 | End: 147 |
| Size | 21 | 24 | 100 |
(AAT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGAAATGGGCATTTGACCC | TCCCATTTCATTTCATGAGATCTGT | Start: 48 |
| Tm | 57.585 | 58.571 | End: 148 |
| Size | 21 | 25 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTGAAATGGGCATTTGACCC | TCCCATTTCATTTCATGAGATCTGTC | Start: 48 |
| Tm | 57.585 | 59.455 | End: 148 |
| Size | 21 | 26 | 101 |
| Primer 3 details |
|---|
| Sequence | TTTGAAATGGGCATTTGACCC | CCCATTTCATTTCATGAGATCTGTCT | Start: 48 |
| Tm | 57.585 | 59.454 | End: 147 |
| Size | 21 | 26 | 100 |
| Primer 4 details |
|---|
| Sequence | TGGTTTCAAGGATATTGGACCCA | ACAAACGAGTACTCCATATTCTCT | Start: 93 |
| Tm | 59.665 | 57.223 | End: 215 |
| Size | 23 | 24 | 123 |
| Primer 5 details |
|---|
| Sequence | TTTGAAATGGGCATTTGACCC | CCCATTTCATTTCATGAGATCTGT | Start: 48 |
| Tm | 57.585 | 57.289 | End: 147 |
| Size | 21 | 24 | 100 |
