ssr_primer
Primer Details for TRINITY_DN11216_c0_g1_i3 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTCCATCAGCAGCAAC | AGGCTCCCAGTGATGAGGAT | Start: 4044 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCCTCCATCAGCAGCAACA | AGGCTCCCAGTGATGAGGAT | Start: 4045 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCCTGGCATCTCGACCATAC | GAGGCTTACTGGGAGAGGGA | Start: 8131 |
| Tm | 59.966 | 60.032 | End: 8293 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | AACCTGCCCAAACTGTCCAA | ACCACCCAAGCTCAAACACA | Start: 2525 |
| Tm | 60.033 | 60.034 | End: 2737 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GTTTGAACAGTGGGCGGAAC | GTCAAGCTGAGCCTCCTGTT | Start: 5238 |
| Tm | 59.97 | 59.964 | End: 5502 |
| Size | 20 | 20 | 265 |
(AT)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTCCATCAGCAGCAAC | AGGCTCCCAGTGATGAGGAT | Start: 4044 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCCTCCATCAGCAGCAACA | AGGCTCCCAGTGATGAGGAT | Start: 4045 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCCTGGCATCTCGACCATAC | GAGGCTTACTGGGAGAGGGA | Start: 8131 |
| Tm | 59.966 | 60.032 | End: 8293 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | AACCTGCCCAAACTGTCCAA | ACCACCCAAGCTCAAACACA | Start: 2525 |
| Tm | 60.033 | 60.034 | End: 2737 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GTTTGAACAGTGGGCGGAAC | GTCAAGCTGAGCCTCCTGTT | Start: 5238 |
| Tm | 59.97 | 59.964 | End: 5502 |
| Size | 20 | 20 | 265 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTCCATCAGCAGCAAC | AGGCTCCCAGTGATGAGGAT | Start: 4044 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCCTCCATCAGCAGCAACA | AGGCTCCCAGTGATGAGGAT | Start: 4045 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCCTGGCATCTCGACCATAC | GAGGCTTACTGGGAGAGGGA | Start: 8131 |
| Tm | 59.966 | 60.032 | End: 8293 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | AACCTGCCCAAACTGTCCAA | ACCACCCAAGCTCAAACACA | Start: 2525 |
| Tm | 60.033 | 60.034 | End: 2737 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GTTTGAACAGTGGGCGGAAC | GTCAAGCTGAGCCTCCTGTT | Start: 5238 |
| Tm | 59.97 | 59.964 | End: 5502 |
| Size | 20 | 20 | 265 |
(TGC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTCCATCAGCAGCAAC | AGGCTCCCAGTGATGAGGAT | Start: 4044 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCCTCCATCAGCAGCAACA | AGGCTCCCAGTGATGAGGAT | Start: 4045 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCCTGGCATCTCGACCATAC | GAGGCTTACTGGGAGAGGGA | Start: 8131 |
| Tm | 59.966 | 60.032 | End: 8293 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | AACCTGCCCAAACTGTCCAA | ACCACCCAAGCTCAAACACA | Start: 2525 |
| Tm | 60.033 | 60.034 | End: 2737 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GTTTGAACAGTGGGCGGAAC | GTCAAGCTGAGCCTCCTGTT | Start: 5238 |
| Tm | 59.97 | 59.964 | End: 5502 |
| Size | 20 | 20 | 265 |
(ATG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTCCATCAGCAGCAAC | AGGCTCCCAGTGATGAGGAT | Start: 4044 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCCTCCATCAGCAGCAACA | AGGCTCCCAGTGATGAGGAT | Start: 4045 |
| Tm | 60.036 | 60.03 | End: 4287 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCCTGGCATCTCGACCATAC | GAGGCTTACTGGGAGAGGGA | Start: 8131 |
| Tm | 59.966 | 60.032 | End: 8293 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | AACCTGCCCAAACTGTCCAA | ACCACCCAAGCTCAAACACA | Start: 2525 |
| Tm | 60.033 | 60.034 | End: 2737 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GTTTGAACAGTGGGCGGAAC | GTCAAGCTGAGCCTCCTGTT | Start: 5238 |
| Tm | 59.97 | 59.964 | End: 5502 |
| Size | 20 | 20 | 265 |
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