Primer Details for TRINITY_DN11177_c3_g15_i1 |
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| (AAAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTAATCAACACTCAACACATTCC | TGTGTGTGTTTGCTTGTGTGA | Start: 90 |
| Tm | 60.05 | 59.117 | End: 336 |
| Size | 25 | 21 | 247 |
| Primer 2 details |
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| Sequence | CGTAATCAACACTCAACACATTCCA | TGTGTGTGTTTGCTTGTGTGA | Start: 91 |
| Tm | 60.05 | 59.117 | End: 336 |
| Size | 25 | 21 | 246 |
| Primer 3 details |
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| Sequence | CGTAATCAACACTCAACACATTCC | TGTGTGTGTTTGCTTGTGTGA | Start: 91 |
| Tm | 58.618 | 59.117 | End: 336 |
| Size | 24 | 21 | 246 |
| Primer 4 details |
|---|
| Sequence | ACGTAATCAACACTCAACACATTCC | TTGTGTGTGTTTGCTTGTGTGA | Start: 90 |
| Tm | 60.05 | 59.705 | End: 337 |
| Size | 25 | 22 | 248 |
| Primer 5 details |
|---|
| Sequence | CGTAATCAACACTCAACACATTCCA | TTGTGTGTGTTTGCTTGTGTGA | Start: 91 |
| Tm | 60.05 | 59.705 | End: 337 |
| Size | 25 | 22 | 247 |
