ssr_primer
Primer Details for TRINITY_DN11163_c2_g1_i1 |
|---|
| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACCACAAAGACCGAGCT | TGCCGACGAGGAAGAAGTTT | Start: 285 |
| Tm | 59.532 | 59.608 | End: 423 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
|---|
| Sequence | CCACATGGAAAATGCCAGTGG | TGCCGACGAGGAAGAAGTTT | Start: 172 |
| Tm | 60.067 | 59.608 | End: 423 |
| Size | 21 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TTCAACCACAAAGACCGAGCT | TGCCGACGAGGAAGAAGTTT | Start: 284 |
| Tm | 60.134 | 59.608 | End: 423 |
| Size | 21 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | CCACATGGAAAATGCCAGTGG | AGCTCGGTCTTTGTGGTTGA | Start: 172 |
| Tm | 60.067 | 59.532 | End: 304 |
| Size | 21 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TCAACCACAAAGACCGAGCT | TGCCGACGAGGAAGAAGTTTT | Start: 285 |
| Tm | 59.532 | 60.203 | End: 423 |
| Size | 20 | 21 | 139 |
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