ssr_primer
Primer Details for TRINITY_DN1115_c0_g1_i1 |
|---|
| (TCT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTTTTTAGGAGCTGGTGG | ACGACCCAGTTGAAGAACAAGA | Start: 73 |
| Tm | 59.195 | 59.83 | End: 320 |
| Size | 20 | 22 | 248 |
| Primer 2 details |
|---|
| Sequence | AGCCTTCGCTTTTTAGGAGCT | ACGACCCAGTTGAAGAACAAGA | Start: 67 |
| Tm | 59.996 | 59.83 | End: 320 |
| Size | 21 | 22 | 254 |
| Primer 3 details |
|---|
| Sequence | GCCTTCGCTTTTTAGGAGCTG | ACGACCCAGTTGAAGAACAAGA | Start: 68 |
| Tm | 59.869 | 59.83 | End: 320 |
| Size | 21 | 22 | 253 |
| Primer 4 details |
|---|
| Sequence | CGCTTTTTAGGAGCTGGTGG | ACGACCCAGTTGAAGAACAAG | Start: 73 |
| Tm | 59.195 | 58.438 | End: 320 |
| Size | 20 | 21 | 248 |
| Primer 5 details |
|---|
| Sequence | CGCTTTTTAGGAGCTGGTGG | GCAAGAAAAACAACAACGACCC | Start: 73 |
| Tm | 59.195 | 59.399 | End: 335 |
| Size | 20 | 22 | 263 |
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