ssr_primer
Primer Details for TRINITY_DN11149_c0_g1_i5 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACTGAGGGCTGGAAGGTG | TCCTTTCCCCAATACCGTGC | Start: 2028 |
| Tm | 59.889 | 60.035 | End: 2286 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
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| Sequence | ATCGATTGCCTATGCTGGGG | AATAAACAATGCAGGCGGCC | Start: 1248 |
| Tm | 59.963 | 59.824 | End: 1382 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
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| Sequence | AAGCTTGGAGTGAGGTTGCA | AATCCATGGGCAGGGTGAAG | Start: 1771 |
| Tm | 59.817 | 60.033 | End: 2006 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
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| Sequence | AATGCGGGCATCTTCCTCAT | AATCCATGGGCAGGGTGAAG | Start: 1829 |
| Tm | 59.816 | 60.033 | End: 2006 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
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| Sequence | CCGCGCTTCCATTAGCAAAG | ACGGGTAGCCTCTGAATCCT | Start: 39 |
| Tm | 60.248 | 60.032 | End: 237 |
| Size | 20 | 20 | 199 |
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